Table 1.
Eighteen different combinations of buffers and detergents used in this studya
| Buffer | Buffer composition | Detergent |
|---|---|---|
| 1 | 50 mM Tris-HCl, 150 mM NaCl, 10% DMSO, 4 mM EDTA | Triton X-100, CHAPS, or NP-40 |
| 2 | 50 mM Tris-HCl, 150 mM NaCl, 10% glycerol, 4 mM EDTA | Triton X-100, CHAPS, or NP-40 |
| 3 | 50 mM Tris-HCl, 150 mM NaCl | Triton X-100, CHAPS, or NP-40 |
| 4 | 25 mM Tris-HCl, 137 mM NaCl, 5 mM KCl, 0.7 mM Na2HPO4 | Triton X-100, CHAPS, or NP-40 |
| 5 | 25 mM Tris-HCl, 25 mM NaCl, 5 mM MgCl2 | Triton X-100, CHAPS, or NP-40 |
| 6 | 150 mM NaCl, 10 mM Tris-HCl, 1 mM EDTA | Triton X-100, CHAPS, or NP-40 |
a
Six different lysis buffers in combination with one of three detergents (1% CHAPS, 0.2% NP-40, and 0.1% Triton X-100) were analyzed for solubilization of membrane-bound r-RVG from Sf-9 cells. One milliliter of each buffer-detergent solution was added to 2 × 107 Sf-9 cells. The pH of each buffer was 7.4.