Table 2.
Optimization of temperature and duration for CHAPS treatmenta
| Duration of treatment | G content (μg/2 × 107 cells) at indicated temp |
||
|---|---|---|---|
| 36–38°C | 23–25°C | 2–8°C | |
| 30 min | 0.88 ± 0.02 | 1.56 ± 0.01 | 1.57 ± 0.01 |
| 1 h | 0.50 ± 0.01 | 1.56 ± 0.02 | 1.53 ± 0.02 |
| 3 h | 0.00 | 1.30 ± 0.02 | 1.23 ± 0.01 |
| 5 h | 0.00 | 1.44 ± 0.02 | 1.31 ± 0.01 |
| 8 h | 0.00 | 1.49 ± 0.01 | 1.21 ± 0.02 |
| 24 h | 0.00 | 1.42 ± 0.02 | 1.28 ± 0.03 |
a
The solubilization of membrane-bound r-RVG from Sf-9 cells by buffer 1 with 0.6% CHAPS was analyzed at different temperatures and for different durations. Three different temperature conditions (2 to 8°C, 23 to 25°C, and 36 to 38°C) and six different incubation periods (30 min, 1 h, 3 h, 5 h, 8 h, and 24 h) were analyzed, and the results are the means ± SDs from three different determinations.