Fig. 2.
Mouse antiserum antibody titration. Serum samples (in binary dilution with an initial dilution of 1:100; performed in triplicate) from mice immunized intraperitoneally with the FaeG-FedF-LT192A2:B fusion protein were titrated for anti-K88, anti-F18, and anti-LT antibodies in ELISAs using purified K88ac fimbriae, F18 fimbriae, and LTB (Sigma) as the coating antigens. HRP-conjugated goat anti-mouse IgG (1:3,300) was used as the secondary antibody. Serum samples collected from the control mice were also included. Antibody titers are expressed at a log10 scale. Bars and error bars represent means and standard deviations. **, P < 0.01.