Table 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| E. coli strains | ||
| TOP 10 | F−mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 nupG | Invitrogen |
| 8963 | pFaeG-FedF-LT192A2:B in TOP 10 | This study |
| 8752 | p8745 in E. coli BL21 | 15a |
| 3030-2 | Porcine ETEC isolate, K88ac/LT/STb | 40 |
| 06-20147 | Porcine ETEC isolate, F18/STa/STb/stx2e | 44 |
| 8516 | Porcine field isolate, F18 | 44 |
| Plasmids | ||
| p8745 | STa13-LT192 fusion cloned in pET28α at NheI and BamHI sites | 15a |
| p8963 | faeG-fedF-eltA2:B fusion in TA cloning pBAD vector | This study |