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. 2011 Oct;18(10):1632–1643. doi: 10.1128/CVI.00411-10

Fig. 11.

Fig. 11.

Determination of chemotaxis in U937 cells. When culture supernatants of HeLa hVECs that were induced with LPS were used in the lower Transwell chamber, U937 cell migration was significantly increased compared to that of the scrambled peptide. When supernatants were obtained from LPS-induced cells treated with increased concentrations of RVFHbαP (70.45, 17.60, and 2.20 μM) and used in the lower Transwell chamber, the migration of U937 cells was inhibited in a dose-dependent manner. RVFHbαP at a concentration of 70.45 μM resulted in maximum inhibition of U937 cell migration. As expected, the scrambled peptide nRVFHbαP did not interfere with LPS-induced chemotaxis. The data were normalized to U937 chemotaxis in response to 0.1 mM fMLP in PBS-BSA, which was used to stimulate cell migration (100%). Each value is the mean ± SD from six individual observations obtained from three independent experiments. Levels of significance (*, P < 0.05; **, P < 0.001; compared with the LPS- and LPS-nRVFHbαP-treated groups) were calculated by an ANOVA test followed by a Bonferroni analysis.