Fig. 5.
Determination of intracellular IL-6 and IL-8 levels in HeLa hVECs. HeLa cells were seeded at a density of 106/well in 24-well plates and treated with LPS (10 μg/ml for 6 h), or LPS-induced (10 μg/ml for 6 h) cells were treated with RVFHbαP (70.45 μM for 1 h) or scrambled peptide (70.45 μM for 1 h). At the end of the treatment, cells were collected, lysed, and used for the determination of IL-6 (a) and IL-8 (b) levels by ELISA as detailed in Materials and Methods. RVFHbαP suppressed these inflammatory mediators induced by LPS compared to the cells that were treated with scrambled peptide. Values represent the means ± SD of triplicate determinations performed on different days. Levels of significance (**, P < 0.001 compared with the LPS- and LPS-nRVFHbαP-treated groups) were calculated by an ANOVA test followed by a Bonferroni analysis.