Skip to main content
. 2011 Oct;18(10):1783–1786. doi: 10.1128/CVI.05174-11

Fig. 1.

Fig. 1.

Immunoblot analysis of M. genitalium cervical antibodies. Cervical results from representative cases and controls (study participant identification numbers listed above) demonstrating the range of reactivity observed against whole-cell M. genitalium G37 lysates. The locations of the immunodominant MgpB (140-kDa) and MgpC (110-kDa) proteins (arrows) were identified using a combination of sera from rabbits (designated “Rb”) immunized with His-tagged recombinant proteins spanning regions of MgpB (amino acids [aa] 1092 to 1209; GenBank accession number AAA25420) or MgpC (aa 36 to 308; GenBank accession number AAA25421). Membranes were exposed for 20 min; however, strongly IgG (participants 123, 197, 208, 303, and 629)- and IgA (participants 123 and 303)-reactive samples were exposed for 2 min to visualize individual bands. Secondary antibody controls in the absence of primary antibody indicated that an unknown M. genitalium protein (∼36 kDa) reacts nonspecifically with anti-human IgG.