Fig. 3.

Confirmation that the dominant M. genitalium antigens are the MgpB and MgpC proteins. The reactivities of MgpB and MgpC (arrows) were confirmed among a subset of immunoblot-positive women by reacting specimens with lysates of wild-type G37 (WT) or mgpB (ΔB) and mgpC (ΔC) deletion mutants. Immunoblot-positive samples failed to react with either antigen in the mutant lysates, because the MgpB and MgpC proteins are reciprocally stabilized; the mgpB mutant has reduced levels of MgpC, and the mgpC mutant has reduced levels of MgpB (1). Representative results are shown above, with the reactivity of participant 95 compared with that of the rabbit anti-MgpB and anti-MgpC control.