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Activity of the ENA1 promoter and relative plasma membrane potential in selected members of group B mutants. The β-galactosidase activity was measured as indicated in Materials and Methods, and data are the means ± standard errors of the means (SEM) of data from five independent experiments performed in triplicate. Plasma membrane potential values, generated from at least three independent experiments, are expressed as a percentage relative to the wild-type value, which was set as 100%.
