Fig. 2.

Expression of the six C. albicans Gap permeases in the S. cerevisiae M4584 strain. Cells transformed with pCaGAPs-GFP plasmids or pScGAP-GFP and empty YEp352, as positive and negative controls, respectively, were first grown to early exponential phase in minimal medium with ammonium sulfate and then transferred to minimal medium supplemented with 100 μg/ml of amino acids and observed in microscope after 4 h of cultivation. Leucine was used for cells transformed with pScGAP1-GFP and pCaGAP6-GFP; phenylalanine was used for cells transformed with pCaGAP2-GFP, pCaGAP3-GFP, and pCaGAP5-GFP; and arginine was used for cells transformed with pCaGAP1-GFP and pCaGAP4-GFP. GFP fluorescence and cells viewed by Nomarski optics are shown.