Fig. 3.

Growth of S. cerevisiae M4584 expressing CaGap permeases on minimal medium with three sources of nitrogen. Tenfold serial dilutions of yeast cell suspensions were prepared, 3 μl was spotted, and plates were incubated for 4 days at 30°C. Cells with empty YEp352 vector and cells expressing S. cerevisiae Gap1 served as negative and positive controls, respectively.