Fig. 4.

Growth of S. cerevisiae cells expressing CaGap permeases on minimal medium with proline (100 μg/ml) and toxic amino acid analogues. Tenfold serial dilutions of yeast cell suspensions were prepared, 3 μl was spotted, and plates were incubated for 4 days at 30°C. Cells with empty YEp352 vector and cells expressing S. cerevisiae Gap1 served as negative and positive controls, respectively. FP, p-fluoro-l-phenylalanine, toxic analogue of phenylalanine; TL, thialysine, toxic analogue of lysine. The M4584 strain was used for experiments with FP, and HS100-3C was used with TL.