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Three CaGap proteins activate the PKA target trehalase in S. cerevisiae. Activation of trehalase by the addition of 10 mM citrulline (A), threonine (B), and methionine (C) to 25.969b cells expressing C. albicans general amino acid permeases and nitrogen starved for 24 h. ▪, ScGap1 (positive control); □, YEp352 (negative control); ○, CaGap1; •, CaGap2; ▵, CaGap3; ▴, CaGap4; ⧫, CaGap5; ⋄, CaGap6.
