Table 1.
Developing buffer | Trajectory speed (μm/min) | Displacement speed (μm/min) | Chemotactic index | Persistency |
---|---|---|---|---|
pH 5.0 | 4.69 ± 0.03 | 3.34 ± 0.03 | 0.56 ± 0.01 | 0.79 ± 0.01† |
pH 6.5 | 5.01 ± 0.02 | 3.06 ± 0.02 | 0.58 ± 0.01 | 0.70 ± 0.01 |
pH 9.0 | 3.89 ± 0.02‡ | 2.07 ± 0.02† | 0.52 ± 0.01 | 0.64 ± 0.01 |
5 mM K+ | 5.01 ± 0.02 | 3.06 ± 0.02 | 0.58 ± 0.01 | 0.70 ± 0.01 |
50 mM K+ | 4.26 ± 0.03 | 1.62 ± 0.03* | 0.30 ± 0.01* | 0.65 ± 0.01 |
The data represent means ± SEM. *, P < 0.001, compared to buffer using 5 mM K+; †, P < 0.01, compared to buffer at pH 6.5; ‡, P < 0.001, compared to buffer at pH 6.5.