Table 2.
Developing buffer | Trajectory speed (μm/min) | Displacement speed (μm/min) | Directedness | Persistency |
---|---|---|---|---|
pH 5.0 | 9.27 ± 0.02*† | 4.79 ± 0.02*† | 0.51 ± 0.01*† | 0.52 ± 0.01*† |
pH 6.5 | 14.79 ± 0.08* | 10.75 ± 0.07* | 0.95 ± 0.01 | 0.73 ± 0.02 |
pH 7.5 | 11.68 ± 0.06† | 9.20 ± 0.06† | 0.97 ± 0.01 | 0.78 ± 0.01 |
pH 9.0 | 9.05 ± 0.02*† | 4.42 ± 0.02*† | 0.30 ± 0.01*† | 0.50 ± 0.01*† |
5 mM K+ | 13.09 ± 0.05 | 8.55 ± 0.04 | 0.93 ± 0.01 | 0.66 ± 0.01 |
25 mM K+ | 6.15 ± 0.03‡ | 3.57 ± 0.02‡ | 0.64 ± 0.01‡ | 0.59 ± 0.01 |
50 mM K+ | 6.46 ± 0.01‡ | 2.95 ± 0.01‡ | 0.24 ± 0.01‡ | 0.46 ± 0.00‡ |
The data represent means ± SEM. *, P < 0.001 compared to buffer at pH 7.5; †, P < 0.001 compared to buffer at pH 6.5; ‡, P < 0.001 compared to that in buffer with 5 mM K+.