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. 2011 Oct;10(10):1357–1366. doi: 10.1128/EC.05041-11

Fig. 3.

Fig. 3.

Bur6, Ncb2, and TBP of C. albicans interact together and bind to the CDR1 promoter fragment in vitro. (A) Coimmunoprecipitation of MBP-Bur6 and MBP-TBP with anti-Ncb2 antibody. Protein combinations used in the experiment are shown at the top of the panels. For blot I, lane 1, MBP-Bur6 and MBP-TBP were subjected to Western blotting (not immunoprecipitated) using anti-MBP antibody. For blot I, lanes 2, 4, 5, and 6, samples first were immunoprecipitated using anti-Ncb2 antibody and then subjected to Western blotting using anti-MBP antibody. For blot I, lane 3, the indicated protein mixture was immunoprecipitated using control immunoglobulin IgG (preimmune) and then subjected to Western blotting using anti-MBP antibody. Blot II, lane 1, blank; lanes 2 to 6, IgG heavy chains of the respective antibodies used in immunoprecipitation reactions. Blot III, lane 1, blank; lanes 2 to 6, Western blot of immunoprecipitated bait protein using anti-Ncb2 antibody. IP, immunoprecipitation; WB, Western blotting. (B) Autoradiogram showing the DNA binding of purified recombinant Ncb2, Bur6, and TBP. (C) Autoradiogram showing the effect of Ncb2, Bur6, and Ncb2-Bur6 on TBP-DNA binding. Protein combinations used in the assay are indicated at the top of the figure.