Fig. 6.

Changes in the frequencies of strains carrying evolved alleles in competition experiments against the progenitor strain. The progenitor strain was set to compete with yeast strains with an MDS3e MKT1e or an MKT1e genotype in a low-glucose environment. In the initial competition mix, the progenitor strain was set as a majority in relation to the strains carrying evolved alleles (100:1). The competition cultures were propagated under either the short-transfer regimen, which aimed to maintain the competing strains under constant fermentative growth (a), or the long-transfer regimen, which aimed to maximize the periods of respiratory growth (b). s values represent the mean selection coefficients of the strains with evolved alleles relative to the progenitor strain. Error bars, ±1 standard error of the mean (n = 3, except for MKT1e in panel b [n = 2]).