Growth of the khd1Δ ccr4Δ and khd1Δ pop2Δ mutant strains. (A) Strain 10BD-c163 that was heterozygous for the khd1Δ and ccr4Δ alleles was sporulated, and the tetrad was dissected onto a YPD plate. Growth after 4 days at 25°C is shown. Genotypes are indicated on the left side. More than 50 tetrads were dissected, and representative data are shown. (B) Strain 10BD-p163 that was heterozygous for the khd1Δ and pop2Δ alleles was sporulated, and the tetrad was dissected onto a YPD plate. Growth after 4 days at 25°C is shown. Genotypes are indicated on the left side. More than 50 tetrads were dissected, and representative data are shown. (C) Strain 10BD-cp163 that was heterozygous for the khd1Δ, ccr4Δ, and pop2Δ alleles was sporulated, and the tetrad was dissected onto YPD containing 10% sorbitol. Growth after 6 days at 25°C is shown. Genotypes are indicated on the both sides. More than 30 tetrads were dissected, and representative data are shown.