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. 2011 Oct;10(10):1340–1347. doi: 10.1128/EC.05181-11

Fig. 3.

Fig. 3.

Overexpression of ROM2 and WSC2 suppresses the growth defect of khd1Δ ccr4Δ double mutants. (A) Multicopy suppressors of khd1Δ ccr4Δ. Transformants of the khd1Δ ccr4Δ strain (c1H-1B) carrying the indicated plasmids were streaked onto YPD medium and incubated at 25°C (left) or 37°C (right). Each patch represents an independent transformant. Plasmids used were YEplac195 (vector), YEplac195-ROM2 (ROM2), and YEplac195-WSC2 (WSC2). (B) Activated Rho1 and Pkc1 alleles in khd1Δ ccr4Δ. Transformants of the khd1Δ ccr4Δ strain (c1H-1B) carrying the indicated plasmids were streaked onto YPD medium and incubated at 25°C (left) or 37°C (right). Each patch represents an independent transformant. Plasmids used were YCplac33 (vector), YCplac-RHO1-Q68L, and YCplac33-PKC1-R398P.