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. 2011 Oct;10(10):1340–1347. doi: 10.1128/EC.05181-11

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Fig. 4. — Khd1 associates with the ROM2 mRNA. Total extracts were prepared from the cells harboring the empty vector, the FLAG-tagged Khd1 plasmid, and the FLAG-tagged Khd1(L284N) plasmid. Khd1-FLAG and Khd1(L284N)-FLAG were immunoprecipitated (IP) with anti-FLAG antibodies. Samples were analyzed by immunoblot analysis with anti-FLAG to test for the presence of Khd1-FLAG proteins. The RNAs were isolated from the total extracts and from the immunoprecipitates. ROM2 and LRG1 mRNAs were detected by RT-PCR.