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. 2011 Oct;10(10):1283–1294. doi: 10.1128/EC.05141-11

Fig. 3.

Fig. 3.

The Paf1 complex colocalizes with actively transcribed SRG1. (A) ChIP analysis of HA-tagged Paf1 complex subunits at SRG1 (SER3-22 and SER3-19) and the flanking AIM9 (SER3-41) and SER3 (SER3-1) genes from untagged (FY4), 3×HA-PAF1 (KY1721), 3×HA-RTF1 (KY2082), LEO1-3×HA (KY785), and 3×HA-CDC73 (KY786) strains grown in YPD at 30°C. (B) ChIP analysis of Ctr9-Myc from untagged (KY399) and CTR9-6×MYC (KY785) strains grown in YPD at 30°C. The relative occupancies of these factors were calculated using qPCR as described in Materials and Methods. Each value represents the mean ± the standard error of the mean of three biological replicates, and asterisks indicate statistical significance compared to the untagged control (*, P < 0.05; **, P < 0.01). Below the graphs is a schematic of the SRG1/SER3 locus, and the arrows indicate the transcription start sites of SRG1 and SER3. The gray box represents the Cha4 binding site, black boxes indicate TATA sequences, and white boxes are sequences required for SER3 activation. The block arrow indicates SRG1 transcription, and the horizontal black bars mark the location of the DNA fragments amplified by qPCR.