Fig. 4.
Effects of coinfection on titan cell formation. Mice were intranasally infected with 2 × 107 wild-type (KN99a or KN99α) or gpr4Δ gpr5Δ mutant cells labeled with Alexa Fluor 488 (Green) or were coinfected with one labeled and one unlabeled strain. Cells obtained by BAL were fixed and examined by microscopy for green fluorescence (cell type) and cell size. More than 300 cells were examined per animal, and the small cells (cell body diameter, <10 μm) and titan cells (cell body diameter, >10 μm) were quantified. Error bars indicate standard deviations for 3 to 4 mice per treatment.