Fig. 1.

Optical maps of ancestral strain WN624 (top), presweep strain WN715 (middle), and postsweep strain WN716 (bottom). Whole genomes of ancestral strain WN624 and evolved strains WN715 and WN716 were digested with NcoI, and optical maps of each strain were created by OpGen technology. The ∼4-kb deletion detected in strain WN716 by optical mapping is indicated by the arrow. All other lines connecting maps are due to small inversions in palindromic regions generated by the mapping program (see Materials and Methods).