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HPLC chromatograms of DMB derivatives of 2-oxo acids. Blank, HPLC-grade water (after derivatization reaction); standard, equal amounts of 2OG, 2OB, and 2OV; sample, cellular extract with the standard 2OB and 2OV. The two visible peaks in the blank overlap with those for some of other tested 2-oxo acids, possibly contamination from the reagents and/or water. Injection of water yields no fluorescence peak.
