Fig. 2.

2OG leakage and steady-state pool concentration during exponential growth of E. coli. Wild-type strain NCM3722 was cultured in NH₄Cl-glycerol medium. (A) 2OG concentrations in the medium were assayed from immediately after inoculation through the late exponential phase of growth. The growth curve is shown in the inset; the doubling time was 62 min. (B) Paired filter and medium samples were collected along the exponential phase of growth. Each pool value was the result of subtraction from paired samples.