Table 1.
Weight gain of limestone prisms for different types of biodeposition treatments
| Stone type (mean % porosity ± SEM) and treatment | pHa |
Weight gain |
||||
|---|---|---|---|---|---|---|
| Step 1 |
Step 2 |
|||||
| Initial | Final | Initial | Final | Absolute (g, %)b | Relative (%, %)b | |
| Less porous | ||||||
| Massangis (9.98 ± 0.66) | ||||||
| Medium | 6.89 | 7.73 | 7.41 | 6.81 | 0.07 ± 0.01 (27) | 0.37 ± 0.04 (25) |
| Bacteria in saline | 6.65 | 7.8 | NAd | NA | 0.00 ± 0.00 (0) | 0.00 ± 0.00 (0) |
| Bacteria in culture liquid | 9.45 | 7.89 | NA | NA | 0.03 ± 0.00 (12) | 0.16 ± 0.02 (11) |
| Biodeposition with supernatant | 9.42 | 9.04 | 7.41 | 6.86 | 0.09 ± 0.02 (34) | 0.46 ± 0.09 (31) |
| Biodeposition with bacteria in saline | 6.65 | 7.8 | NA | 5.5 | 0.22 ± 0.03 (83) | 1.11 ± 0.13 (74) |
| Biodeposition with bacteria in culture liquid | 9.43 | NDc | ND | ND | 0.27 ± 0.01 | 1.49 ± 0.04 |
| Aubigny (14.12 ± 0.34) | ||||||
| Medium | 6.89 | 7.72 | 7.41 | 6.77 | 0.12 ± 0.01 (22) | 0.71 ± 0.07 (24) |
| Bacteria in saline | 6.65 | 7.85 | NA | NA | 0.00 ± 0.00 (1) | 0.02 ± 0.01 (1) |
| Bacteria in culture liquid | 9.45 | 7.11 | NA | NA | 0.06 ± 0.01 (11) | 0.38 ± 0.05 (13) |
| Biodeposition with supernatant | 9.42 | 8.74 | 7.41 | 6.73 | 0.21 ± 0.03 (38) | 1.29 ± 0.16 (43) |
| Biodeposition with bacteria in saline | 6.65 | 7.85 | NA | 5.54 | 0.39 ± 0.03 (71) | 2.18 ± 0.18 (72) |
| Biodeposition with bacteria in culture liquid | 9.43 | ND | NA | ND | 0.55 ± 0.02 | 3.02 ± 0.13 |
| Euville (17.24 ± 0.59) | ||||||
| Medium | 6.89 | 7.69 | 7.41 | 7.52 | 0.11 ± 0.02 (16) | 0.67 ± 0.14 (20) |
| Bacteria in saline | 6.65 | 7.85 | NA | NA | 0.00 ± 0.01 (0) | 0.00 ± 0.03 (0) |
| Bacteria in culture liquid | 9.45 | 7.58 | NA | NA | 0.05 ± 0.01 (8) | 0.33 ± 0.05 (10) |
| Biodeposition with supernatant | 9.42 | 8.99 | 7.41 | 6.98 | 0.17 ± 0.02 (26) | 1.03 ± 0.10 (30) |
| Biodeposition with bacteria in saline | 6.65 | 7.85 | 7.41 | 5.8 | 0.39 ± 0.04 (61) | 2.15 ± 0.24 (63) |
| Biodeposition with bacteria in culture liquid | 9.43 | ND | ND | ND | 0.64 ± 0.08 | 3.40 ± 0.29 |
| More porous | ||||||
| Savonnières ( 30.92 ± 0.14) | ||||||
| Medium | 6.89 | 7.6 | 7.41 | 6.79 | 0.20 ± 0.03 (30) | 1.48 ± 0.20 (33) |
| Bacteria in saline | 6.65 | 7.8 | NA | NA | 0.00 ± 0.00 (0) | 0.00 ± 0.03 (0) |
| Bacteria in culture liquid | 9.45 | 7.65 | NA | NA | 0.08 ± 0.01 (13) | 0.63 ± 0.12 (14) |
| Biodeposition with supernatant | 9.42 | 9.11 | 7.41 | 6.93 | 0.20 ± 0.02 (31) | 1.49 ± 0.13 (33) |
| Biodeposition with bacteria in saline | 6.65 | 7.8 | 7.41 | 5.74 | 0.63 ± 0.05 (97) | 4.73 ± 0.40 (105) |
| Biodeposition with bacteria in culture liquid | 9.43 | ND | NA | ND | 0.65 ± 0.01 | 4.51 ± 0.05 |
| Avesnes (32.10 ± 0.05) | ||||||
| Medium | 6.89 | 7.68 | 7.41 | 6.78 | 0.22 ± 0.01 (36) | 1.74 ± 0.15 (40) |
| Bacteria in saline | 6.65 | 7.65 | NA | NA | −0.01 ± 0.00 (−1) | −0.07 ± 0.02 (−2) |
| Bacteria in culture liquid | 9.45 | 7.97 | NA | NA | 0.10 ± 0.02 (16) | 0.71 ± 0.08 (16) |
| Biodeposition with supernatant | 9.42 | 8.97 | 7.41 | 6.63 | 0.31 ± 0.06 (51) | 2.32 ± 0.25 (53) |
| Biodeposition with bacteria in saline | ND | ND | ND | ND | ND | ND |
| Biodeposition with bacteria in culture liquid | 9.43 | ND | ND | ND | 0.61 ± 0.01 | 4.34 ± 0.05 |
a
Columns 2 to 5 indicate the evolution of the pH during the different steps of the biodeposition treatment. Porosity was determined from water saturation under vacuum.
b
Percent weight gain of prisms treated by biodeposition of bacteria in culture liquid. Values are means ± the standard errors of the means.
c
ND, not determined.
d
NA, not applicable.