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HpyF3I restriction patterns of the amplified flaB DNA (604 or 598 bp) from Borrelia strains. Lanes: M, marker DNA (Nova 100-bp DNA ladder); 1, 9, and 12, B. valaisiana; 2, 3, 6, and 10, B. afzelii; 4 and 5, B. lusitaniae; 7 and 8, B. miyamotoi; 11, B. bissettii. Positive controls: B. afzelii VS461 (lane 10), B. bissettii DN127 (lane 11), and B. valaisiana VS116 (lane 12).
