Table 1.
Borrelia strains used in this study for working out the PCR-RFLP protocol based on the flaB gene for differentiation of Borrelia species and strains transmitted by I. ricinus
| Borrelia species | Strain | Source | Countrya (reference) | RFLP patternb with: |
Accession no. | |
|---|---|---|---|---|---|---|
| HpyF3I | Ecl136II | |||||
| B. burgdorferi sensu stricto | DG-1 | Canis lupus familiaris (blood) | Poland (21) | I | Ia | DQ016625 |
| D69-04 | I. ricinus | Poland (19) | I | Ia | DQ016620 | |
| ZS7 | I. ricinus | Germany | I | Ia | NC_011728 | |
| GL56-07 | I. ricinus | Poland† | I | Ia | HM345910 | |
| T90-5-02 | I. ricinus fed on Turdus merula | Poland† | I | Ia | HM345911 | |
| Borrelia sp. SV1 | SV1 | I. ricinus | Finland | I | Ib | NZ_ABJZ02000005 |
| B. afzelii | 9W10-04 | I. ricinus fed on Capreolus capreolus | Poland (19) | II | FJ874924 | |
| VS461 | I. ricinus | Switzerland | II | D63365 | ||
| ZL109-07 | I. ricinus | Poland† | II | HM345907 | ||
| ST19-05 | I. ricinus | Poland† | II | HM345908 | ||
| OS17-07 | I. ricinus | Poland† | II | HM345909 | ||
| P-Gau | Human | Germany | III | X63413 | ||
| B. bissettii | DN127 | Ixodes pacificus | United States | IV | D82857 | |
| B. garinii | 20047 | I. ricinus | France | V | Va | D82846 |
| PB35-99 | I. ricinus | Poland† | V | Va | HM345897 | |
| DB60-01 | I. ricinus | Poland† | V | Va | HM345899 | |
| ZL148-07 | I. ricinus | Poland† | V | Va | HM345900 | |
| D7-04 | I. ricinus | Poland (19) | V | Vb | DQ016622 | |
| D106-04 | I. ricinus | Poland (19) | V | Vb | DQ016621 | |
| T32-5-05 | I. ricinus fed on C. capreolus | Poland (19) | V | Vb | DQ650336 | |
| DB1F7-04 | I. ricinus | Poland (19) | V | Vb | DQ650331 | |
| DK29 | Human | Denmark | V | Vb | X69608 | |
| Far04 | Fratercula arctica | Faroe Islands | V | Vb | NZ_ABPZ02000016 | |
| HE | V | Vb | X69609 | |||
| K48 | I. ricinus | Slovakia | V | Vb | X69610 | |
| KL10 | V | Vb | L42881 | |||
| PBr | Human | Germany | V | Vb | NZ_ABJV02000003 | |
| DB74-01 | I. ricinus | Poland† | V | Vb | HM345898 | |
| T44-4-02 | I. ricinus fed on Turdus philomelos | Poland† | V | Vb | HM345904 | |
| ST12-05 | I. ricinus | Poland† | V | Vb | HM345905 | |
| RP54-05 | I. ricinus | Poland† | V | Vb | HM345906 | |
| T53-9-02 | I. ricinus fed on T. merula | Poland† | V | Vd* | HM345901 | |
| T40-10-02 | I. ricinus fed on T. philomelos | Poland† | V | Vd* | HM345902 | |
| T41-2-02 | I. ricinus fed on T. philomelos | Poland† | V | Vd* | HM345903 | |
| B. bavariensis sp. nov. | PBi | Human | Germany | V | Vc | NC_006156 |
| TRO | Human | Slovenia | V | Vc | X69614 | |
| DB18N6-04 | I. ricinus | Poland† | V | Vc | DQ650333 | |
| B. valaisiana | BA9F9-05 | I. ricinus | Poland (19) | VI | DQ650330 | |
| D58-04 | I. ricinus | Poland (19) | VI | DQ016624 | ||
| VS116 | I. ricinus | Switzerland | VI | D82854 | ||
| T107-7-02 | I. ricinus fed on T. merula | Poland† | VI | HM345913 | ||
| PR45-05 | I. ricinus | Poland† | VI | HM345912 | ||
| B. lusitaniae | D23-04 | I. ricinus | Poland (19) | VII | DQ016623 | |
| DB8-09-04 | I. ricinus | Poland† | VII | HM345916 | ||
| SP6-09 | I. ricinus | Poland† | VII | HM345914 | ||
| SP38-09 | I. ricinus | Poland† | VII | HM345915 | ||
| PotiB2 | I. ricinus | Portugal | VIII | VIIIa | D82856 | |
| B. spielmanii | A14S | Human | Netherlands | VIII | VIIIb | NZ_ABKB02000003 |
| B. miyamotoi | WL10-6-04 | I. ricinus fed on C. capreolus | Poland (19) | IX | DQ650338 | |
| 123T05-2 | I. ricinus fed on Cervus elaphus | Poland (19) | IX | FJ823229 | ||
| D110-07 | I. ricinus | Poland (20) | IX | FJ518804 | ||
| LB-M56 | I. ricinus | France | IX | AF529084 | ||
| OS109-07 | I. ricinus | Poland† | IX | HM345917 | ||
| PB111-09 | I. ricinus | Poland† | IX | HM345918 | ||
| SP48-09 | I. ricinus | Poland† | IX | HM345919 | ||
| ZL27-07 | I. ricinus | Poland† | IX | HM345920 | ||
a
†, this study.
b
*, new restriction pattern obtained in this study.