Table 3.
Kinetic parameters of F. johnsoniae strain A3 for various substrates tested at 15°C
| Substrate | Kinetic model | Kinetic parameter |
R2 | ||
|---|---|---|---|---|---|
| Vmax (h−1)a | Ks (μg C liter−1 ± SD) | Specific affinity (×10−2 liter·μg−1 of C·h−1 ± SD) | |||
| Fructose | Monod | 0.22 | 34.4 (± 1.1) | 0.69 (± 0.01) | 0.99 |
| Teissier | 0.19 | 24.0 (± 0.6) | 0.81 (± 0.01) | 0.99 | |
| Maltose | Monod | 0.33 | 14.4 (± 0.1) | 2.3 (± 0.002) | 0.99 |
| Teissier | 0.29 | 11.5 (± 0.1) | 2.5 (± 0.02) | 1.00 | |
| Laminarin | Monod | 0.29 | 3.4 (± 0.3) | 8.5 (± 0.5) | 0.95 |
| Teissier | 0.27 | 3.4 (± 0.2) | 7.9 (± 0.01) | 0.98 | |
| Amylopectin | Monod | 0.23 | 17.0 (± 0.2) | 1.4 (± 0.01) | 0.99 |
| Teissier | 0.20 | 14.3 (± 1.3) | 1.4 (± 0.05) | 0.98 | |
| Xyloglucan | Monod | 0.24 | 21.8 (± 1.4) | 1.1 (± 0.05) | 0.99 |
| Teissier | 0.21 | 17.8 (± 0.8) | 1.2 (± 0.04) | 0.99 | |
| Proline | NDb | 0.019 | <2.5b | >0.76b | |
| Gelatin | Monod | 0.22 | 18.5 (± 0.1) | 1.2 (± 0.01) | 0.99 |
| Teissier | 0.19 | 14.5 (± 0.04) | 1.3 (± 0.01) | 1.00 | |
a
Standard deviation ≤0.005 h−1 for all Vmax values determined.
b
Actual Ks and specific affinity could not be determined (ND), because Vmax had already been reached at the lowest ΔS of 2.5 μg C liter−1.