Fig. 1.
Microcrystalline cellulose conversion assay. HiCDH was loaded at 1 mg protein per gram cellulose, TaGH61A was loaded at 5 mg protein per gram cellulose, and HjCBH-I, HjEG-II, and AoBG were loaded at 10 mg protein per gram cellulose. Assay conditions were 50 mM NaOAc at pH 5, 1 mM MnSO4, 50°C, and a 72-h time course. Monosaccharide and disaccharide were quantified by RID-HPLC using an Aminex HPX-87H column, with percent cellulose conversion calculated compared to a limit digest by a high loading of cellulase. Error bars are for triplicate cellulose cleavage reactions, each measured once.