Table 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| K1060a | F−fadE62 lacI60 tyrT58(AS) fabB5 mel-1 | 32 |
| KQ1 | Same as K1060, carrying pQEKm and pBBR1MCS; Kmr Cmr | This work |
| K24K1 | Same as K1060, carrying pJP24K and pBBR1MCS; Kmr Cmr | 9 |
| K24KP | Same as K1060, carrying pJP24K and pADP; Kmr Cmr | 9 |
| MC4100a | araD139 Δ(argF-lac)U19 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | 6 |
| ADA100 | Same as MC4100, but λφ(ibp::lacZ) | 3 |
| ADAQ1 | Same as ADA100, carrying pQEKm and pBBR1MCS; Kmr Cmr | This work |
| ADAK1 | Same as ADA100, carrying pJP24K and pBBR1MCS; Kmr Cmr | This work |
| ADAKP | Same as ADA100, carrying pJP24K and pADP; Kmr Cmr | This work |
| ADA1001 | Same as ADA100, carrying pBBR1MCS; Cmr | This work |
| ADA100P | Same as ADA100, carrying pADP; Cmr | This work |
| Plasmids | ||
| pQEKm | Expression vector; ColE1 ori; Apr Kmr | 30 |
| pJP24K | pQEKm derivative containing the phaBAC genes from Azotobacter sp. strain FA8 under the control of a T5 promoter/lac operator element; Kmr | 30 |
| pBBR1MCS | Broad host range; lacPOZ′ mob RP4; Cmr | 23 |
| pADP | pBBR1MCS carrying phaP from Azotobacter sp. FA8; Cmr | 9 |
a
Strain obtained through the E. coli Genetic Stock Center, Yale University, New Haven, CT.