Table 2.
Primers used for qRT-PCR analysis
| Gene | Primer pair | Description | Reference |
|---|---|---|---|
| groEL | 5′-GTAACGACGCTCGTGTGAAA-3′ | Chaperone; its expression is regulated by RpoH. | 12 |
| 5′-GCAACGGAAACACCATCTTT-3′ | |||
| groES | 5′-AATGGCCGTATCCTTGAAAA-3′ | Chaperone; its expression is regulated by RpoH. | 12 |
| 5′-AATTGCCAGAATGTCGCTTT-3′ | |||
| dnaK | 5′-GAACCCGCAAAACACTCTGT-3′ | Chaperone; its expression is regulated by RpoH. | 12 |
| 5′-GTGCCATTTTCTGGCCTTTA-3′ | |||
| ibpA | 5′-AATCTGCTGGTGGTGAAAGG-3′ | Small heat shock protein; its expression is regulated by RpoH. | 1 |
| 5′-ACCAGGTTAGCACCACGAAC-3′ | |||
| rpoH | 5′-GGCTGAAAAGCTGCATTACC-3′ | Alternative sigma factor σH; it controls the heat shock response. | 12 |
| 5′-ATTACCATGGCGATCTGGAA-3′ | |||
| rpoE | 5′-GTCGTCCACCTTCCAGTGAT-3′ | Alternative sigma factor σE; it controls the responses to heat shock and other stresses on membrane and periplasmic proteins. | 27 |
| 5′-TAAATCTTCCGGGAGGGACT-3′ | |||
| rpoS | 5′-CGGAGTTGAGGTTTTTGACG-3′ | Alternative sigma factor σS; master regulator of the general stress response | 17 |
| 5′-GGCCGTTAACAGTGGTGAAT-3′ | |||
| dps | 5′-TGCTTTATACCCGCAACGAT-3′ | Protects against different types of stresses; its expression is regulated by RpoS. | 2 |
| 5′-GAAGCCATCCAGCATTTCAT-3′ | |||
| poxB | 5′-ATCGCCAAAACACTCGAATC-3′ | Pyruvate oxidase; its expression is regulated by RpoS. | 7 |
| 5′-CTCCGCTAAGTTGTGCTTCA-3′ | |||
| yfiD | 5′-AACTCTTTCTGGCTGCTGGA-3′ | Stress-induced alternate pyruvate formate-lyase subunit | 44 |
| 5′-ACCTTCAACGCGAACTTCTG-3′ | |||
| rrsB | 5′-GTGCTACAATGGCGCATACA-3′ | 16S rRNA | 29 |
| 5′-GGCATTCTGATCCACGATTA-3′ |