Table 3.

Biomass, PHB, and protein concentrations for 12-h shaken flask cultures of strains KQ1 (control strain), K24K1 (PHB-producing strain), and K24KP (PHB- and PhaP-producing strain)

E. coli strain	CDWa (g·liter −1)	PHB (g·liter−1)	% PHB (wt/wt)b	Soluble protein concnc (g·liter−1)	Soluble protein concn/CDW
KQ1	9.20 ± 0.07	NDd	ND	2.7 ± 0.6	0.29 ± 0.13
K24K1	11.60 ± 0.06	2.4 ± 0.1	21 ± 1	1.7 ± 0.3	0.15 ± 0.09
K24KP	12.23 ± 0.08	3.0 ± 0.3	27 ± 2	3.5 ± 0.5	0.28 ± 0.13

^aCDW, cell dry weight.

^bThe amount of PHB is given as a percentage of the CDW (average ± standard deviation).

^cSoluble proteins were extracted by sonication and centrifugation, and the amounts of proteins were determined by the Bradford assay as described in Materials and Methods.

^dND, not determined.