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. 2011 Sep;77(18):6663–6673. doi: 10.1128/AEM.05111-11

Fig. 2.

Fig. 2.

PCR amplification of the hyvI and hyvII gene target regions in isolates of “Candidatus Liberibacter asiaticus” from Florida and other global origins, using primer sets LJ788/LJ729 for hyvI (A) and LJ812/LJ1089 for hyvII (B). Lane M: 1-kb DNA ladder (Promega); lanes 1 to 16, DNA from “Ca. Liberibacter asiaticus”-infected plants or psyllids of global origins outside the United States; lanes 17 to 38, DNA from “Ca. Liberibacter asiaticus”-infected different hosts in Florida; lanes 17 to 19, DNA from “Ca. Liberibacter asiaticus”-positive psyllid from field; lane 20, DNA from “Ca. Liberibacter asiaticus”-free psyllid control from greenhouse; lanes 20 to 23, DNA from “Ca. Liberibacter asiaticus”-positive citrus from field; lanes 24 to 26, DNA from “Ca. Liberibacter asiaticus”-positive citrus from greenhouse, CG8-S/CG8-R leaf samples collected from scion/rootstock of grafted citrus CG8; lane 27, “Ca. Liberibacter asiaticus”-free citrus control from greenhouse; lanes 28 to 32, “Ca. Liberibacter asiaticus”-positive periwinkle from greening, B1 and B2 from branch 1 and branch 2 in dodder-transmitted periwinkle plants P1 (lanes 28 and 29) and PP11 (lanes 30 and 31), PG from grafted-transmitted periwinkle plant; lane 33, “Ca. Liberibacter asiaticus”-free periwinkle control; lanes 34 to 37, “Ca. Liberibacter asiaticus”-positive dodder collected from citrus, periwinkle, tobacco, and potato; lane 38, “Ca. Liberibacter asiaticus”-free dodder control.