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Kinetic plots of the P. acidilactici rhamnosidase Ram2 with pNP-rhamnopyranoside (▾) and rutinose (•). The data were interpreted with the Hill equation (24); nh values were 2.0 ± 0.1 and 1.0 ± 0.1 for pNP-rhamnopyranoside and rutinose, respectively, and 100% relative activity refers to Vmax. All determinations were performed in triplicate; the error bars indicate standard deviations (SD).
