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Oligonucleotides used for amplification of rhamnosidase genes ram and ram2 from P. acidilactici
| Primer name | Sequence (5′-3′)a |
|---|---|
| ramF (NdeI) | GATATACATATGAATCAAACTTATTGGATC |
| ramR (XhoI) | GTGGTGCTCGAGCGCAATGGGAATTACTACG |
| ram2F (BsaI)b | GGTCTCACATGGCATTTACATTTCAAA |
| ram2R (XhoI) | GTGGTGCTCGAGAGATAAATACTTTCTCATTAAATA |
Restriction sites are underlined.
Digestion with BsaI creates an overhang for ligation with the NcoI site of pET21d.
