Table 3.
Influences of solvents, sugars, and salts in the assay on activities of the P. acidilactici rhamnosidases Ram and Ram2 determined with pNP-α-l-rhamnopyranoside (1 mM)
| Additivea | Relative enzyme activity (%)b |
|
|---|---|---|
| Ram | Ram2 | |
| Solvents | ||
| EtOH (6% [vol/vol]) | 56 ± 0.5 | 78 ± 0.8 |
| EtOH (12% [vol/vol]) | 31 ± 1 | 74 ± 0.7 |
| DMSO (12.5% [vol/vol]) | 37 ± 0.7 | 11 ± 0.7 |
| DMSO (25% [vol/vol]) | 24 ± 1 | 4.7 ± 0.2 |
| Sugars | ||
| Rhamnose (250 mM) | 15 ± 1 | 45 ± 1 |
| Glucose (250 mM) | 76 ± 1 | 2.4 ± 0 |
| Salts | ||
| NaCl (100 mM) | 130 ± 2 | 121 ± 1 |
| KCl (100 mM) | 128 ± 6 | 142 ± 3 |
| K2SO4 (100 mM) | 115 ± 1 | 113 ± 6 |
| MgSO4 (100 mM) | 84 ± 2 | 111 ± 3 |
| MgCl2 (100 mM) | 127 ± 5 | 120 ± 2 |
| CaCl2 (100 mM) | 123 ± 1 | 102 ± 3 |
a
EtOH, ethanol.
b
Activity in the absence of additives (standard assay conditions) was set at 100%. All data represent the average of triplicate determination ± SD.