Table 1.
Enzymatic activities associated with acetone and butanol production
| Enzyme | Activity (U/mg) in: |
|
|---|---|---|
| Strain N1-4 | Coculturea | |
| CoA-transferase (acetate) | 29.72 ± 2.43 | 27.43 ± 2.51 |
| CoA-transferase (butyrate) | 28.64 ± 2.55 | 29.36 ± 2.75 |
| Acetoacetate decarboxylase | 140.81 ± 17.14 | 168.08 ± 23.70 |
| Butyraldehyde dehydrogenase | 1.24 ± 0.01 | 0.95 ± 0.13 |
| Butanol dehydrogenase | 1.04 ± 0.07 | 0.90 ± 0.02 |
a
Activities were normalized based on thiolase activity because crude extracts were prepared from cocultured strains N1-4 and C. thermocellum.