Fig. 1.
Genomic context of llmg_pseudo_10 of L. lactis MG1363 and its relation to the homologous regions (hr's) present in pSEUDO and pSEUDO-GFP. The multiple cloning site (MCS) contains, in clockwise order, EcoRI, XmaI/SmaI, SphI, ScaII, SalI, HindIII, BglII, XhoI, and BamHI restriction enzyme sites. The gfp gene for superfolder GFP (17) was cloned in pSEUDO using the XhoI and BamHI sites, yielding pSEUDO-GFP. Terminators are indicated by “lollipop” structures. The vertical line on llmg_pseudo_10 depicts the stop codon that prematurely halts translation of the gene in L. lactis MG1363. eryR, erythromycin resistance gene. oroP encodes the L. lactis orotate transporter (3).