Table 1.
Results of various PCR and serological assays for Stx and stx subtypesa
| Strain | Serotype | Stx subtype(s) | 5P |
BAM |
V-R |
STAT |
AB (stx) | PrE (Stx) | PrST (Stx) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| stx1 | stx2 | stx1 | stx2 | Stx1 | Stx2 | Stx1 | Stx2 | ||||||
| AA1 | O174:H8 | 1c, 2b | + | + | + | − | + | − | + | − | + | + | + |
| BB2 | O55:H7 | 1a | + | − | + | − | + | − | + | − | + | + | + |
| CC3 | O128ac[H2] | 2f | − | − | − | − | − | + | − | − | − | − | − |
| DD4 | O177:[H25] | 2c, 2d | − | + | − | + | − | + | − | + | + | + | + |
| EE5 | O111:[H8] | 1a, 2a | + | + | + | + | + | + | + | + | + | + | + |
| FF6 | O113:H4 | 1c, 2b | + | + | + | − | + | + | + | − | + | + | + |
| GG7 | O103:H2 | 1a | + | − | + | − | + | − | + | − | + | + | + |
| HH8 | O26:H11 | 1a | + | − | + | − | + | − | + | − | + | + | + |
| II9 | O41:H26 | 1d | − | − | − | − | + | − | + | − | − | + | + |
| JJ10 | O157:H7 | 2c | − | + | − | + | − | − | − | − | + | − | − |
| 05622 | O138b | 2e | − | + | − | + | − | − | − | − | + | − | − |
| B2F1 | O91:H21b | 2d | − | + | − | + | − | + | − | + | + | + | + |
| D3509 | O2:H25c | 2g | − | + | − | + | − | − | − | − | − | − | − |
a
Assays: 5P, multiplex PCR; BAM, Bacteriological Analytical Manual RT-PCR; V-R, VTEC-RPLA; STAT, ImmunoCard STAT! lateral flow device; AB, seropathotype A/B PCR; PrE, Premier EHEC ELISA; PrST, ProSpectT Shiga toxin (E. coli) microplate assay ELISA.
b
Strains were obtained from the STEC Center, Michigan State University.
c
Strain was obtained from the Staten Serum Institute.