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Construction of pJFW027. A linear DNA fragment containing the entire sequence of pJFW017 was generated by PCR amplification using primers JF266 and JF267 and ligated into the origin fragment indicated in Fig. 1, also generated by PCR amplification using primers JF268 and JF282. Plasmids containing the various origin fragments described in the legend of Fig. 1 were cloned into pJFW017 for testing.
