Figure 1.

Macrophages surround cysts in murine models of PKD. Kidneys were perfusion fixed with 4% PFA and cryosections stained for macrophage marker F4/80. (A) Cyst-lining cells showing closely apposed macrophages stained green. Green, F4/80; Red, DBA. (B) Quantitative analysis of FACS-sorted macrophages defined as F4/80⁺CD45⁺CD11c^- cells and further analyzed for Ly6c expression; ***P < 0.0001. (C) Cystic area from Pkd2^WS25/- kidney showing several F4/80⁺ cells (green). Inset shows magnified view of area marked with an asterisk. (D) Noncystic area from Pkd2^ws25/+ kidney showing very few F4/80⁺ cells. Inset shows magnified view of selected area indicated by the asterisk. (E) Quantitative analysis of macrophages in kidneys from Pkd2^WS25/- and Pkd2^ws25/+ (control) mice. (F) Transwell migration of macrophages toward DMEM-F12, DMEM-F12 + 10%FBS, DMEM-F12 conditioned medium from Pkd1^fl/- cells, and DMEM-F12 conditioned media from Pkd1^-/- cells, respectively, n = 3. Inset: qRT-PCR for Mcp-1 and Cxcl16 was performed on RNA from Pkd1^fl/- and Pkd1^-/- cells and reported as the fold increase of Pkd1^-/- as compared with Pkd1^fl/-; n = 3.