Figure 3.

csCSF-1 and spCSF-1 increase intrarenal Mø and promote activation resulting in Mø mediated TEC apoptosis. (A) Number of intrarenal CD68⁺ cells (Mø, DC) and T cells (CD4⁺) in TgC/+, TgCS/+, TgSPP/+, and TgSGP/+ mice with advancing lupus nephritis (1.5, 3.0, and 5.0 months of age). The values are means ± SEM. (B) Mø (CD11b⁺) activation (CD86⁺, CD69⁺, and TNF-α) and T cell activation (CD4⁺; CD86⁺/CD69⁺) in the kidney analyzed by flow cytometry in TgCS/+, TgSPP/+, TgSG/+, wild-type (WT), and Csf1^op/op mice at 3.0 and 5 months of age. The data are representative of three separate experiments. (C) TEC apoptosis by anti-cleaved caspase-3 staining for apoptosis and TEC using morphologic criteria is increased in TgCS/+ and TgSPP/+ mice compared with TgSGP/+ and Csf1^op/op mice. The values are the means ± SEM.