Figure 4.

csCSF-1, spCSF-1 and sgCSF-1 heightened bone-marrow monocyte proliferation, but only csCSF-1 and spCSF-1 shift circulating Mø to the inflammatory phenotype. (A) Proliferation of BM CD11b⁺ leukocytes evaluated by flow cytometry 24 h after intraperitoneal administration of Brdu to TgCS/+, TgSPP/+, TgSGP/+, wild-type (WT), and Csf1^op/op mice. (B and C) Flow cytometric analysis of circulating SSC^lowCD11b⁺Ly6C^hi, Ly6Ci^int, and Ly6C^lo leukocytes in BM, blood, and kidney. The mice were 5 months of age. The values are the means ± SEM. Representative FACS plots are shown.