Figure 4.

Renal p-AMPK is reduced with HFD at 1 week and increased with AICAR. Representative photomicrographs of p-AMPK (green) staining in glomeruli from mice on a (A) STD, (B) HFD, or (C) HFD + AICAR for 1 week. Nuclei were stained using DAPI (blue color). Semiquantitative analysis of p-AMPK-positive staining per glomerular area (D), and quantitative analysis of p-AMPK level in the protein extractions from renal cortex (E) at week 1. Values are means ± SEM. n = 6 in each group. Statistical analyses were performed by one-way ANOVA followed by Newman–Keuls test. *P ≤ 0.05 versus mice on STD; #P ≤ 0.05 versus mice on HFD.