Figure 3.

Bone marrow-derived fibroblast precursor accumulates in obstructed kidneys in a time-dependent manner. (A) Representative cytometric diagrams showing CD45- and collagen-I-positive fibroblast precursors in the kidney of WT mice with or without UUO for 3, 5, or 7 days. Freshly isolated renal cells were stained with CD45 and collagen I and analyzed with flow cytometry. (B) Quantitative analysis of CD45 and collagen I dual-positive fibroblast precursors in the kidney of WT mice with or without UUO for 3, 5, or 7 days as determined by flow cytometry. **P < 0.01. n = 3 per group.