Table 2.
Glycerol-induced sporulation of wild-type and btkA mutant strainsa
| Strain | Incubation (h) | Total no. of spores induced | No. of viable spores |
|---|---|---|---|
| Wild type | 3 | 6.5 × 107 ± 1.1 × 107 | 5.5 × 102 ± 1.0 × 102 |
| btkA mutant | 3 | 4.6 × 107 ± 0.7 × 107 (71)b | 0 (0) |
| Wild type | 24 | 14.0 × 107 ± 3.8 × 107 | 2.1 × 104 ± 1.0 × 104 |
| btkA mutant | 24 | 9.8 × 107 ± 1.0 × 107 (70) | 1.0 × 102 ± 0.2 × 102 (0.5) |
a
The total numbers of spores were counted with a hemocytometer. After sonication for 1 min and heat treatment (15 min at 50°C), the numbers of viable spores were determined by plating serial dilutions on CTT agar plates and incubating them at 27°C. The data are the means ± standard errors of triplicate determinations.
b
Values in parentheses are percentages relative to the values for the wild type.