Table 2.
PCR primers used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| ExoAT132-NdeI-F | GATCTACATATGAAAAGCCCGCATCGACTTTGCGTC |
| ExoAT132-PstI-R | GATCTTCTGCAGTTTTTATTTAGTCACACCGGTTTGCAGCACGCTGTCC |
| ExoATC3-NcoI-F | GATCCCATGGAAAGCCCGCATCGACTTTGCGTCGCCAAGAG |
| ExoATC3-SbfI-R | CTAGCCTGCAGGGCTCAAAGTTCCTCGCTTTTTATTTAGTCACG |
| ExoAT11-NdeI-F | GATCTACATATGATTGTGCCCGATAAGCCGCCACAG |
| ExoAT11-PstI-R | GATCTACTGCAGTCACGAGCCGCGAAACCACGTCAGCAG |
| NasY2-NdeI-F | GATCTTCATATGTCTCTACCTGCTTACCACTCGAATCC |
| NasY2-PstI-R | GATCTACTGCAGCCAATCAAGACAAAGCAATAGCAAACCTTGTTC |
| NasR-NdeI-F | GATCATCATATGCAGCCAGAGATCTTCGCGCTTCGTTATG |
| NasR-PstI-R | GATCTTCTGCAGTCTCAGTCGCTCACATCTCGCCGCGGAAC |
a
Relevant restriction sites are in boldface.