Fig. 11.

Verification of the insensitivity of a pknB mutant to carolacton by determination of CFU. CFU of carolacton-treated (2.5 μg/ml or 0.25 μg/ml) and untreated (control) biofilm cells of a pknB-deficient strain (A) and the wild type (B) are shown. Cells derived from 3 different wells of the microtiter plate were diluted independently, and each dilution was plated out on 3 agar plates, resulting in 9 different values for each condition. The standard deviation from these 9 technical replicates is shown. Each experiment was repeated twice, and the figure shows a representative result.