Fig. 2.

Elution of Ado, MTA, and Ino cleavage activities from M. smegmatis cell extract applied to the HiTrap Q anion-exchange column. M. smegmatis cell extract was applied to the HiTrap Q anion-exchange column and eluted with 150 to 750 mM NaCl gradient. Every other fraction was tested for Ade formation from Ado or MTA and Hx formation from Ino by the xanthine oxidase assay. The formation of Ade and Hx is expressed as the percent change in initial absorbance at 305 nm (for Ado and MTA) and 293 nm (for Ino) after a 2-hour incubation with Ado, MTA, and Ino.